Part:BBa_K5086010
hsa-mir-99b_DDSD
hsa-mir-99b_DDSD (BBa_K50860010) is a single-stranded DNA sequence with 70 nucleotides that is a self-enhancing probe for the target hsa-mir-99b_Rep. The sequence at its 3' end is designed to be complementary to the Universalness_h strand, so that it binds specifically to the Universalness_h strand and thus accomplishes recognition of the input signal. DNA polymerase polymerizes DNA in the 5'-3' direction from the 3' end of the target hsa-mir-99b_Rep. During DNA polymerization by Bst DNA polymerase, the wt strand is replaced and released by the newly synthesized daughter strand, which also releases a single strand of DNA whose sequence is identical to that of the target (the target strand). At the same time, a single strand of DNA with the same sequence as the target strand (U to T) will be released, which will serve as the target for the first step of the polymerase-mediated strand displacement reaction to realize the DDSD self-enhancement process, and the wt strand will be involved in the subsequent reaction to produce the fluorescence report.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |